The association between cyclooxygenase-2 (COX-2/PTGS2) gene polymorphism and osteoarthritis gene polymorphism and osteoarthritis gene polymorphism and osteoarthritis
Volkan G. Güler1, Serap Yalın1, Mehmet Berköz2, Özlem Bölgen Çimen3, Erdinç Yalın4, Pelin Eroğlu1, Arzu Kanık5
1Departments of Biochemistry
2Pharmaceutical Technology, Mersin University Pharmacy Faculty, Mersin, Turkey
3Physical Medicine and Rehabilitation, 5Biostatistics, Medical Faculty of Mersin University, Mersin, Turkey
4Departments of Biochemistry, Medical Faculty of Çukurova University, Adana, Turkey
5Biostatistics, Medical Faculty of Mersin University, Mersin, Turkey
Keywords: COX-2; osteoarthritis; polymorphism.
Abstract
Objectives: This study aims to investigate the relationship between the risk for the development of osteoarthritis and Cyclooxygenase-2 (COX 2) -765G>C gene polymorphism.
Patients and methods: We included a total of 100 osteoarthritis patients (18 males, 82 females; mean age 60.4±8.4 years; range 41 to 81 years) who were treated in the Physical Therapy Clinic and 100 healthy subjects without a history of arthritis (40 males, 60 females; mean age 30.9±7.5 years; range 16 to 48 years) in our study between September 2006 and May 2008. The frequency of -765G>C gene polymorphism in the COX-2 promoter region was investigated in the osteoarthritis patients and the control group without a history of arthritis using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The data were analysed with chi-square and logistic regression analysis.
Results: The frequencies of -765G>C polymorphism for GG, GC and CC genotypes were found to be 54%, 35%, and 11% in the control group and 48%, 34% and 18% in the osteoarthritis group, respectively.
Conclusion: Based on the data obtained, it can be stated that there is no significant relation between COX-2 -765G>C polymorphism and osteoarthritis disease. Furthermore, this study presents the first results of COX-2 promoter variant in Turkish patients with osteoarthritis.