THE EFFECT OF THE APPLICATION OF FOREIGN BODY ON LOCAL AND SYSTEMIC FINDINGS OF EXPERIMENTAL OSTEOMYELITIS IN MICE
Volkan ÖZTUNA1, Gülden ERSÖZ2, Banu COŞKUN3, Ali KAYA2, Mehmet ÇOLAK1, Fehmi KUYURTAR1
1Mersin Üniversitesi Tıp Fakültesi Ortopedi ve Travmatoloji Anabilim Dalı
2Mersin Üniversitesi Tıp Fakültesi Klinik Bakteriyoloji ve İnfeksiyon Hastalıkları Anabilim Dalı
3Mersin Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı
Keywords: Osteomyelitis, Animal Model, Foreign Body.
Abstract
Introduction: In this study, by inducing acute hematogenous and post-traumatic osteomyelitis in mice, the effect of the foreign body fitted into bone on local infection findings and other organ involvement were investigated. Materials and
Method: Thirty mice used in this study were separated into five groups. In all of the subjects the medullary canals were destroyed and the first group formed the control. In the 2nd and the 3rd groups hematogenous osteomyelitis were developed using meticilline resistant Staphylococcus aureus, and in the 3rd group a piece of silicon drain tube was placed into the medullary canal. In the 4th and the 5th groups post-traumatic osteomyelitis were induced using meticilline resistant Staphylococcus aureus and in the 5th group a piece of silicon drain tube was placed into the medullary canal. Ten days later mice were killed and tibias were studied by macroscopically, microbiologically, and histopathologically. Blood samples were inoculated in agar plate. Samples taken from the liver, the spleen, the kidney, the heart muscle, the lung, and the brain tissues were observed histopathologically. Findings of the group 2 versus 3 and the group 4 versus 5 were statistically compared by using SPSS 9.05 program.
Results: There was no local or systemic finding of infection in the control group. The application of foreign body in hematogenous and post-traumatic osteomyelitis models did not influence local or systemic infection findings.
Conclusion: Our findings suggest that, the process of bacteria settlement in bone is independent from foreign bodies present in the environment.