Diabetes mellitus accelerates fatty degeneration of the supraspinatus muscle after tendon tear: An experimental study in rats

Baver Acar; Özkan Köse; Güneş Aytaç; Serra Öztürk; Asiye Kübra Göksu; Gamze Tanrıover; Muzaffer Sindel

doi:10.5606/ehc.2018.62388

Baver Acar¹, Özkan Köse¹, Güneş Aytaç², Serra Öztürk², Asiye Kübra Göksu³, Gamze Tanrıover³, Muzaffer Sindel²

¹Department of Orthopedics and Traumatology, University of Health Sciences, Antalya Training and Research Hospital, Antalya, Turkey
²Department of Anatomy, Akdeniz University Faculty of Medicine, Antalya, Turkey
³Department of Histology and Embryology, Akdeniz University Faculty of Medicine, Antalya, Turkey

Keywords: Diabetes mellitus; fatty degeneration; oil red O; peroxisome proliferator-activated receptor gamma; rotator cuff tear; supraspinatus muscle.

Abstract

Objectives: This study aims to evaluate the effect of diabetes mellitus (DM) on intramuscular fatty degeneration after a fullthickness supraspinatus (SS) tendon tear in rats. Materials and methods: The study included 24 adult male Wistar Albino rats (age, 18 to 24 weeks; weighing 320-380 g) randomized into a sham group (n=6), control group (n=6) and experimental group (n=12). Rats with fasting blood glucose levels ?250 mg/dL at each measurement after an injection of streptozotocin were accepted to have DM. On the seventh day of the study, the SS muscles of the rats in the experimental and control groups were cut from the insertion. All animals were performed euthanasia four weeks after the surgical procedure and SS muscles were excised completely. Fatty degeneration in the SS muscle was assessed histologically and immunohistochemically with oil red O and peroxisome proliferatoractivated receptor gamma (PPAR-g) staining using histological score (H-score) and quantitative methods.
Results: More intense oil red O and PPAR-g staining was observed in all regions of the SS muscles of the experimental group compared to control and sham groups (p<0.05).
Conclusion: The results of this study showed that DM accelerates intramuscular fatty degeneration after SS tendon tears. Fatty degeneration should be monitored closely in diabetic patients with rotator cuff tear who were selected for conservative treatment and early surgical treatment should be considered as an option.