NITRIC OXIDE AND MALONDIALDEHYDE LEVELS IN OSTEOARTHRITIS
Erhan YILMAZ1, Seval YILMAZ1, Lokman KARAKURT1, Erhan SERİN2
1Fırat Üniversitesi Tıp Fakülesi Ortopedi ve Travmatoloji Anabilim Dalı, Elazığ
2Fırat Üniversitesi Veteriner Fakülesi Biyokimya Anabilim Dalı, Elazığ
Keywords: Osteoarthritis, Synovial fluid level, Nitric oxide, Lipid peroxidation, Malondialdehyde.
Abstract
Introduction: Nitric oxide and oxygen radicals has been considered as an important mediator in inflammatory phases and in loss of cartilage. The objective of this study was to evaluate the nitric oxide and oxygen radical levels in the synovial fluid of osteoarthritic knee.
Patients and methods: Synovial fluid was collected during the knee arthroplasty of 15 cases with osteoarthritis (group I) and during the diagnostic knee arthroscopy of 15 cases (group II) without osteoarthritis. Mean age was 57 years in group I and 26 years in group II. Synovial fluid level of nitric oxide was measured spectrophotometrically utilizing the Griess reaction and synovial fluid level of malondialdehyde level was measured spectrophotometrically utilizing the Satoh method. The synovial fluid levels of nitric oxide and malondialdehyde of groups were compared.
Results: Synovial fluid levels of nitric oxide and malondialdehyde in group I were found higher than group II, and this difference was statistically significant (p<0.01 for nitric oxide, p<0.001 for malondialdehyde).
Discussion: Cytokines induce nitric oxide and reactive oxygen radicals synthesis by stimulating the synovial and chondral tissue metabolism. By inhibition of these radicals synthesis, chondral tissue damage and osteoarthritis may be prevented.