Estrogen receptor is involved in the osteoarthritis mediated by Atg16L1-NLRP3 activation
Fa-Xue Liao1,2*
, Shuo Yang1,2*, Zhi-Hong Liu1,2, Kai-Da Bo1,2, Peng-Fei Xu1,2, Jun Chang1,2
1Department of Orthopedics, the First Affiliated Hospital, Anhui Medical University, Hefei, China
2Anhui Public Health Clinical Center, Hefei, China
Keywords: Atgl6L1, estrogen receptor, interleukin-1 beta, NLRP3, osteoarthritis.
Abstract
Objectives: This study aims to explore the mechanisms of dual regulation of osteoarthritis (OA) progression by the involvement of estrogen receptor (ER) in autophagy and inflammation.
Materials and methods: Bioinformatics methods were used to explore the relationship among associated genes. Western blot assays were used to detect related protein expression of OA in C28I2 and induced OA cellular model. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis were used to detect OA related gene expression in C28I2 and induced OA cellular model. Co-immunoprecipitation (CO-IP) analysis were used to verify the direct interaction between ER and NOD-like receptor thermal protein domain associated protein 3 (NLRP3).
Results: The C28I2 cellular model of OA was induced by interleukin-1β (IL-1β). The small interfering ribonucleic acid (SiRNA)-mediated knockdown of autophagy-related 16 like 1 (Atg16L1) in C28I2 decreased the expression of MAP1LC3B (LC3B) and NLRP3. Besides, ER-beta (ERβ) agonist changed the gene expression of NLRP3 and Atg16L1. Moreover, CO-IP analysis indicated the direct interaction between ER and NLRP3.
Conclusion: Our study results revealed that Atgl6L1, NLRP3, and IL-1β interacted closely and ERβ was involved in OA process by affecting autophagy and inflammatory activation.
* The two authors contributed equally to this study
Citation: Liao FX, Yang S, Liu ZH, Bo KD, Xu PF, Chang J. Estrogen receptor is involved in the osteoarthritis mediated by Atg16L1- NLRP3 activation. Jt Dis Relat Surg 2024;35(3):i-viii. doi: 10.52312/ jdrs.2024.1247.