Topical or intravenous administration of tranexamic acid accelerates wound healing
Sönmez Sağlam1
, Zafer Orhan2, Mehmet Arıcan2, Yalçın Turhan2, Zekeriya Okan Karaduman2, Yıldıray Tekçe3, Mücahid Osman Yücel4
1Department of Orthopedics and Traumatology, Gülhane Training and Research Hospital, Ankara, Türkiye
2Department of Orthopedics and Traumatology, Düzce University Faculty of Medicine, Düzce, Türkiye
3Department of Orthopedics and Traumatology, Gebze State Hospital, Kocaeli, Türkiye
4Department of Orthopedics and Traumatology, Yenikent State Hospital, Sakarya, Türkiye
Keywords: Intravenous, local, rat, tranexamic acid, wound healing.
Abstract
Objectives: In this study, we aimed to investigate the morphological and histological effects of tranexamic acid (TA) on wound healing in a rat wound model.
Materials and methods: A total of 24 adult male Wistar Albino rats were used in this study. All rats were simple randomly divided into three groups including eight rats in each group. A full-thickness skin defect was created on the back of the rats in all groups. Serum physiological (2 mL) was instilled saline drops after wound formation (control group). Wound was created and topical TA (0.12 to 0.15 mL [30 mg/kg]) was applied (local group). Intravenous TA (0.12 to 0.15 mL [30 mg/kg]) was applied intravenously before the wound was created (intravenous group). The wound diameters of the groups were photographed and measured on Days 0, 3, 7, 10, 14 and, at the end of Day 14, the rats were sacrificed and their histopathological results and wound diameters were compared.
Results: Fibroblast count values of the control group were found to be significantly lower than the local group (p=0.002), and no significant difference was observed between the local and intravenous groups (p>0.05). The collagen density (%) values of the control group were found to be significantly higher than the local and intravenous groups (p=0.016 and p=0.044). Wound diameter values of the control group on Day 10 day were found to be significantly higher than the local and intravenous groups (p=0.001). In addition, the wound diameter values of the control group on Day 14 were found to be significantly higher than the local and intravenous groups (p=0.001 and p=0.0001). The wound diameter changes of the control group on Days 0-10 were found to be significantly lower than the local and intravenous groups (p=0.001). In addition, the wound diameter changes of the control group on Days 0-14 were found to be lower than those of the local and intravenous groups (p=0.001 and p=0.0001).
Conclusion: The use of local or intravenous TA may have positive effects on the fibroblast count and wound contraction in a rat wound model.
Citation: Sağlam S, Orhan Z, Arıcan M, Turhan Y, Karaduman ZO, Tekçe Y, et al. Topical or intravenous administration of tranexamic acid accelerates wound healing. Jt Dis Relat Surg 2024;35(1):85-95. doi: 10.52312/jdrs.2023.1044.
Animal care was carried out with the prior approval of the Düzce University Animal Experimental Ethics Committee on 19 February 2019 with approval number 2019/1/7 and was in full compliance with Turkish Law 6343/2, Veterinary Medicine Deontology Regulation 6.7.26, and the Helsinki Declaration of Animal Rights.
Idea/concept, literature review, critical review, references and fundings: M.A.; Design, writing the article, materials: M.A., S.S.; Control/supervision: Z.O., Z.O.K.; Data collection: Y.T.; Processing: M.O.Y.; Analysis and/ or interpretation: M.A., Y.T.
The authors declared no conflicts of interest with respect to the authorship and/or publication of this article.
The authors received no financial support for the research and/or authorship of this article.
The data that support the findings of this study are available from the corresponding author upon reasonable request.