Abdullah Alper Şahin1, Erdem Değirmenci2, Kutay Engin Özturan3, Tülin Fırat4, Aysel Kükner5

1Department of Orthopedics and Traumatology, Ordu University Training and Research Hospital, Ordu, Turkey
2Department of Orthopedics and Traumatology, Private Cagsu Hospital, Düzce, Turkey
3Department of Orthopedics and Traumatology, AIBU Izzet Baysal Training and Research Hospital, Bolu, Turkey
4Department of Histology and Embryology, AIBU Izzet Baysal Training and Research Hospital, Bolu, Turkey
5Department of Histology and Embryology, Near East University, Lefkoşa, Turkish Republic of Northern Cyprus

Keywords: Acellular matrix scaffold, adipose tissue, osteochondral defect, stem cell, stromal vascular fraction


Objectives: This study aims to evaluate the effect of adipose-derived stromal vascular fraction (SVF) on osteochondral defects treated by hyaluronic acid (HA)-based scaffold in a rabbit model.

Materials and methods: Eighteen white New Zealand rabbits were randomly grouped into the experimental group (n=9) and control group (n=9). In all groups, osteochondral defects were induced on the weight-bearing surfaces of the right femoral medial condyles, and a HA-based scaffold was applied to the defect area with microfractures (MFs). In this study, 1 mL of adipose-derived SVF was injected into the knee joints of the rabbits in the experimental group. For histological and macroscopic evaluation, four rabbits were randomly selected from each group at Week 4, and the remaining rabbits were sacrificed at the end of Week 8. Macroscopic assessments of all samples were performed based on the Brittberg scoring system, and microscopic evaluations were performed based on the O’Driscoll scores.

Results: Samples were taken at Weeks 4 and 8. At Week 4, the O’Driscoll scores were significantly higher in the control group than the experimental group (p=0.038), while there was no significant difference in the Brittberg scores between the two groups (p=0.108). At Week 8, the O’Driscoll score and Brittberg scores were statistically higher in the experimental group than in the control group (p=0.008 and p=0.007, respectively). According to the microscopic evaluation, at the end of Week 8, the cartilage thickness was greater in the experimental group, and nearly all of the defect area was filled with hyaline cartilage.

Conclusion: Application of adipose-derived SVF with MF-HA-based scaffold was better than MF-HA-based scaffold treatment in improving osteochondral regeneration. Therefore, it can be used in combination with microfracture and scaffold to accelerate cartilage regeneration, particularly in the treatment of secondary osteoarthritis.

Citation: Şahin AA, Değirmenci E, Özturan KE, Fırat T, Kükner A. Effects of adipose tissue-derived stromal vascular fraction on osteochondral defects treated by hyaluronic acid-based scaffold:Anexperimentalstudy.JtDisRelatSurg2021;32(2):347-354.

Conflict of Interest

The authors declared no conflicts of interest with respect to the authorship and/or publication of this article.

Financial Disclosure

The authors received no financial support for the research and/or authorship of this article.